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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Cancer Biol Ther. 2009 Oct;8(19):1806–1814. doi: 10.4161/cbt.8.19.9427

Figure 3.

Figure 3

Claudin-4 promotes HUVEC angiogenesis. A. Pictures show HUVEC cultures following incubation with conditioned media from HOSE-B cells expressing claudin-4 (CLDN4-2) or non expressing control-tranfected cells (VECTOR-1). HUVEC incubated with claudin-4 expressing cells show an increase in tube formation. B. Tube formation was quantitated by counting the number of branch points in a given frame. The graph shows the average of 3 experiments. C. Effects of claudin-4 expression on HUVEC migration. The graph shows the migration (fluorescence levels) following claudin-4 expression at four different time points. The data shown is the average of 3 independent experiments. Media from claudin-4 expressing cells lead to a significant increase in HUVEC migration.