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. Author manuscript; available in PMC: 2009 Dec 17.
Published in final edited form as: Cancer Biol Ther. 2009 Oct;8(19):1840–1851. doi: 10.4161/cbt.8.19.9440

Figure 3.

Figure 3

KLF4 binds to the human Notch1 promoter and activates transcription. (A) Schematic of the human Notch1 promoter (-9903 to +240 bp relative to the ATG initiating codon). Consensus KLF4 binding sites (RRGGYGY) are indicated by vertical lines, and sites conserved in mouse and rat are indicated (*). A broken arrow indicates the putative start site of transcription. Chromatin immunoprecipitation (ChIP) fragments K to P and their sizes in bp are indicated. (B) ChIP analysis was performed on MCF10A/HA-KLF4 cells using the indicated antibodies. (C) Fragments 1 to 3 of the human Notch1 promoter were inserted into the luciferase reporter pGL3-Pro. (D) Relative transcription of Notch1 promoter constructs co-transfected into HEK293 cells with either KLF4 expression vector or control. Three experiments were performed, each in triplicate. standard deviation (SD) bars are shown.