Skip to main content
. Author manuscript; available in PMC: 2009 Dec 17.
Published in final edited form as: Cancer Biol Ther. 2009 Oct;8(19):1840–1851. doi: 10.4161/cbt.8.19.9440

Figure 4.

Figure 4

Transformation by KLF4 requires active Notch1. (A) To determine whether processing of Notch1 to the active form (N1IC) contributes to malignant transformation by KLF4, RK3E cells were transduced with N1IC or KLF4-ER retroviruses and cultured in the presence/absence of γsI. The experiment was performed twice in triplicate. (B) KLF4-ER cells were transfected with Notch1-specific siRNAs, and N1IC expression was determined by immunoblot 72 h later. A portion of the filter was queried in parallel with β-tubulin antibody (loading control). (C) RK3E cells were transfected with Notch1-specific siRNAs and then transduced with KLF4-ER retrovirus 24 h later. Transformed foci were scored at 20 d. ErbB2 served as a control oncogene and showed no inhibition by siRNas. Three independent experiments were performed in duplicate. (D) Quantification of transformed foci from the experiment shown in panel C and not shown (ErbB2). Bars, SD.