Rn Notch1 promoter- BamHI (S) |
5′ AGAGATCTTCGCCAATGGAGGCACACAGGCAG 3′ |
Rn Notch1 promoter- REcoRI (AS) |
5′ GGAATTCGAAGGTCGGTCCTCCCTGAT 3′ |
hsnotchATG (S) |
5′ CAGCCGGTGGGGAGGCTAACCGCCGCTCCTGGC3′ |
hsnotchATG (AS) |
5′ GCCAGGAGCGGCGGTTAGCCTCCCCACCGGCTG 3′ |
hsnotch740ATG (S) |
5′ GCGGGGAGGCCAGCATCTAGAGGGAAAAGCG 3′ |
hsnotch740ATG (AS) |
5′ CGCTTTTCCCTCTAGATGCTGGCCTCCCCGC 3′ |
|
Oligonucleotides used for ChIP analysis |
K (S) |
5′ GGCCCTGACACAGGCTGGCTTCC 3′ |
K (AS) |
5′ GGTTCGTCTGGGTCACAAACGTCCC 3′ |
L (S) |
5′ AGCCCCGCGCTTCCTTCTATGGA 3′ |
L (AS) |
5′ CCTGGC CACCTCTTGCCAAATGC 3′ |
M (S) |
5′ CCTGGCACACCTCTTGCCAAATGC 3′ |
M (AS) |
5′ TTCTCCACCACGATGCCAGGCACG 3′ |
N (S) |
5′ GGCACTGGTCCCGGCTGCTCCCT 3′ |
N (AS) |
5′ GAGCAGCTGAGGCCACGTTGGGG 3′ |
O (S) |
5′ TGGGCTCGGGACGCGCGGCTCAG 3′ |
O (AS) |
5′ GCTCCGCGCCCGGCTCGTTCCTT 3′ |
P (S) |
5 ′ GCTCAGCTCGGGGAGGCGCAAAG 3 ′ |
P (AS) |
5 ′ GAAGGTCGGTCCTCCCTGAT 3 ′ |
|
Oligonucleotides used for semiquantitative RT-PCR |
Rn DLL1 (S) |
5 ′ TGACAAGAGCAGCTTTAAGGCCCG 3 ′ |
Rn DLL1 (AS) |
5 ′ CTGTTTCTCAGCAGCAGTCCCTGG 3 ′ |
Rn DLL3 (S) |
5 ′ GCTGACTCACAGCGCTTCCTTCT 3 ′ |
Rn DLL3 (AS) |
5 ′ GTCGGTGTGGGGCAGGGATTGGA 3 ′ |
Rn DLL4 (S) |
5 ′ TGTGGGCAGCCGCTGCGAGTTTC 3 ′ |
Rn DLL4 (AS) |
5 ′ GTAACCGAAGTGGCACCTTCTCTCC 3 ′ |
Rn Jagged1 (S) |
5 ′ CAGACAGTGCGTGCCGCCATAGGT 3 ′ |
Rn Jagged1 (AS) |
5 ′ CAGCAACTGCTGACATCAAATCCCC 3 ′ |
Rn Jagged2 (S) |
5 ′ TCTGCTCTGGAATCCGAGCCCTG 3 ′ |
Rn Jagged2 (AS) |
5 ′ TTGTTGGCGCTCTCGTCCCGTGG 3 ′ |
Rn GAPDH (S) |
5 ′ TACTAGCGGTTTTACGGGCG 3 ′ |
Rn GAPDH (AS) |
5 ′ TCGAACAGGAGGAGCAGAGAGAGCGA 3 ′ |