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. Author manuscript; available in PMC: 2009 Dec 17.
Published in final edited form as: Cancer Biol Ther. 2009 Oct;8(19):1840–1851. doi: 10.4161/cbt.8.19.9440

Table 2.

Sequences of DNA oligonucleotides used in this study

Name Olignucleotides used for cloning
Rn Notch1 promoter- BamHI (S) 5′ AGAGATCTTCGCCAATGGAGGCACACAGGCAG 3′
Rn Notch1 promoter- REcoRI (AS) 5′ GGAATTCGAAGGTCGGTCCTCCCTGAT 3′
hsnotchATG (S) 5′ CAGCCGGTGGGGAGGCTAACCGCCGCTCCTGGC3′
hsnotchATG (AS) 5′ GCCAGGAGCGGCGGTTAGCCTCCCCACCGGCTG 3′
hsnotch740ATG (S) 5′ GCGGGGAGGCCAGCATCTAGAGGGAAAAGCG 3′
hsnotch740ATG (AS) 5′ CGCTTTTCCCTCTAGATGCTGGCCTCCCCGC 3′
Oligonucleotides used for ChIP analysis
K (S) 5′ GGCCCTGACACAGGCTGGCTTCC 3′
K (AS) 5′ GGTTCGTCTGGGTCACAAACGTCCC 3′
L (S) 5′ AGCCCCGCGCTTCCTTCTATGGA 3′
L (AS) 5′ CCTGGC CACCTCTTGCCAAATGC 3′
M (S) 5′ CCTGGCACACCTCTTGCCAAATGC 3′
M (AS) 5′ TTCTCCACCACGATGCCAGGCACG 3′
N (S) 5′ GGCACTGGTCCCGGCTGCTCCCT 3′
N (AS) 5′ GAGCAGCTGAGGCCACGTTGGGG 3′
O (S) 5′ TGGGCTCGGGACGCGCGGCTCAG 3′
O (AS) 5′ GCTCCGCGCCCGGCTCGTTCCTT 3′
P (S) 5 ′ GCTCAGCTCGGGGAGGCGCAAAG 3 ′
P (AS) 5 ′ GAAGGTCGGTCCTCCCTGAT 3 ′
Oligonucleotides used for semiquantitative RT-PCR
Rn DLL1 (S) 5 ′ TGACAAGAGCAGCTTTAAGGCCCG 3 ′
Rn DLL1 (AS) 5 ′ CTGTTTCTCAGCAGCAGTCCCTGG 3 ′
Rn DLL3 (S) 5 ′ GCTGACTCACAGCGCTTCCTTCT 3 ′
Rn DLL3 (AS) 5 ′ GTCGGTGTGGGGCAGGGATTGGA 3 ′
Rn DLL4 (S) 5 ′ TGTGGGCAGCCGCTGCGAGTTTC 3 ′
Rn DLL4 (AS) 5 ′ GTAACCGAAGTGGCACCTTCTCTCC 3 ′
Rn Jagged1 (S) 5 ′ CAGACAGTGCGTGCCGCCATAGGT 3 ′
Rn Jagged1 (AS) 5 ′ CAGCAACTGCTGACATCAAATCCCC 3 ′
Rn Jagged2 (S) 5 ′ TCTGCTCTGGAATCCGAGCCCTG 3 ′
Rn Jagged2 (AS) 5 ′ TTGTTGGCGCTCTCGTCCCGTGG 3 ′
Rn GAPDH (S) 5 ′ TACTAGCGGTTTTACGGGCG 3 ′
Rn GAPDH (AS) 5 ′ TCGAACAGGAGGAGCAGAGAGAGCGA 3 ′