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Dependence of C75 ionization on Mg2+ concentration. HDV crystals at pH 6.0 show a Mg2+ concentration-dependent change of Raman intensity of neutral C75 peak (1528 cm−1). The y-axis is the ratio of Raman intensity of neutral C75 at 1528 cm−1 to the intensity of adenine ring mode at 725 cm−1 (internal standard). The intensity of the latter is independent of Mg2+ concentration.
