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. Author manuscript; available in PMC: 2011 Jan 4.
Published in final edited form as: FEBS Lett. 2010 Jan 4;584(1):99–105. doi: 10.1016/j.febslet.2009.11.006

Fig. 3.

Fig. 3

Effect of AA-tRNA domain changes on product yields using the GUU mutant anticodon. (A) mRNA sequences. (B) Yields from single incorporations of the test substrates. (C) Yields from five straight incorporations of the test substrates. Note that because the anticodon change in the test substrates (blue) required codon changes in the mRNAs, this in turn required using a very different wild-type substrate (green) as a positive control for these mRNAs. The Ala-tRNA3Ala wild-type substrate also gave similar yields (the green points in Figs. S1 and 2); this positive control is more informative because it only differs from the left-hand test substrate by the lack of modifications and the anticodon change. For simplicity, the Ala-tRNA3Ala positive control is not replotted here but the “-mods” and “AC” differences are written adjacent to the left arrow.