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Steps for constructing the mixed monolayer of FcC11SH and encapsulated HRP at a Au electrode: (a) formation of a mixed monolayer of MPA/FcC11SH, (b) activation of and NHS ester attachment onto MPA, (c) cross-linking of HRP, and (d) encapsulation of HRP through acryloylation. For illustration, the molecules are not drawn to scale.
