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. Author manuscript; available in PMC: 2010 Dec 22.
Published in final edited form as: Biochemistry. 2009 Dec 22;48(50):11929–11938. doi: 10.1021/bi901775j

FIGURE 8.

FIGURE 8

Reconstitution of the Brh2/Dss1 complex. Reaction mixtures containing Brh2 apoprotein were mixed with His-Dss1. After 1 hr at 4°, Ni2+-NTA beads were added, washed, then eluted with SDS sample buffer (80 μL). Aliquots (10 μL) of the supernatant (first wash) and bound fractions were separated by SDS gel electrophoresis. After staining with SimplyBlue Safestain, bands were quantitated using the Odyssey detection platform. Lane a -- supernatant, no Dss1; lane b -- bound, no Dss1; lane c -- supernatant, plus Dss1; lane d -- bound, plus Dss1; lane e -- 12.5% of total Brh2 apoprotein initially added to the reaction. M--size standards.