TABLE 1.
MSCVneo-TLX1 Integration Sites in Immortalized Cell Lines
| Cell line | Gene at integration sitea | Gene ID | Chromosome | LTR junction | Location and distanceb |
Retrovirus orientation |
|
|---|---|---|---|---|---|---|---|
| HX1 | Abcb9 | ATP-binding cassette sub-family B member 9 |
56325 | mu5qF | 124501873 | 3’, 10 kb | R |
| HX2 | Foxl1 | forkhead box L1 | 14241 | mu8qE1 | 123707088 | 3’, 56 kb | R |
| HX3 | Lrrc33 | leucine rich repeat containing 33 | 224109 | mu16qB2 | 32164358 | intron 1, 21 kb | F |
| HX3 c32 | Lrrc33 | leucine rich repeat containing 33 | 224109 | mu16qB2 | 32164358 | intron 1, 21 kb | F |
| HX4 | Gpr39 | G protein-coupled receptor 39 | 71111 | mu1qE3 | 127573910 | 5’ UTR, <1 kb | R |
| Akap13 | A kinase (PRKA) anchor protein 13 | 75547 | mu7qD2 | 82728485 | intron 5, 26 kb | R | |
| Lyrm7 | LYR motif containing 7 | 75530 | mu11qB1.3 | 54621217 | 3’, 19 kb | R | |
| Itm2a | integral membrane protein 2A | 16431 | muXqD | 104681297 | 5’, 89 kb | R | |
| HX4 c4 | Thtpa | thiamine triphosphatase | 105663 | mu14qC3 | 55714396 | exon 1, <1 kb | F |
| HX4 c13 | Itsn1 | intersectin 1 (SH3 domain protein 1A) |
16443 | mu16qC4 | 91794474 | intron 5, 12 kb | R |
| HX5 | Lmo2 | LIM domain only 2 | 16909 | mu2qE2 | 103800793 | 5’, 10 kb | R |
R, reverse; F, forward.
a
MSCVneo-TLX1 insertion within gene or nearest gene; four MSCVneo-TLX1 integration sites were recovered for the HX4 cell line.
b
Location and distance of MSCVneo-TLX1 integration site with respect to transcription start site of gene (identified using UCSC table browser).