TABLE 3.
Expression of Genes Surrounding MSCVneo-TLX1 Integration Sites
| Gene | Cell line-specific integration |
Expressiona | Detection method |
|---|---|---|---|
| Abcb9 | HX1 | + | RT-PCR |
| Vps37b | − | Northern | |
| Pitpnm2 | − | Northern | |
| Foxl1 | HX2 | − | RT-PCR |
| Foxc2 | − | RT-PCR | |
| Lrrc33 | HX3 / HX3 c32 | − | Northern |
| Tnk2b | + | qRT-PCR | |
| Gpr39 | HX4 | − | Northern |
| Ap1g2 | HX4 c4 | − | Northern |
| Itsn1 | HX4 c13 | − | Northern |
| Lmo2 | HX5 | + | Northern |
| Fbxo3 | + | RT-PCR | |
a
No differential expression was observed for any gene near or containing an MSCVneo-TLX1 insertion compared to the other cell lines lacking the insertion; + or −, indicates whether mRNA was detectable or below detection, respectively, by the method used.
b
Tnk2 has 3 transcription start sites located ~481 kb, 504 kb, and 509 kb away from the MSCVneo-TLX1 integration site in HX3 and HX3 c32 cells; its expression in HX3 cells was initially detected by genome-wide expression analysis (I. Riz and R.G. Hawley, unpublished observations).