Table 1. Estimated Densities of Fos-ir Neurons in the Saline Control Groups.

	Fos-ir densitya
	Basal	SAS 1 day	SAS 6 days
Cortex
Frontal association	+	++	+
Orbital	+++	+++	+++
(Piriform)	++	+++	+++
(Prelimbic)	+	++	++
Primary motor	++	++	++
Granular insular	+	++	++
(Anterior cingulate)	+	++	+++
(Primary somatosensory)	+	+++	+++
Dysgranular insular	++	++	++
Dorsal hippocampus	tr	+	+
Ventral hippocampus	ND	ND	+
Subiculum	ND	ND	++
Granular retrosplenial	++	+++	+++
Agranular retrosplenial	++	+++	++
Primary auditory	++	+++	+++
Primary visual	ND	+++	+++
Perirhinal	+	++	++
Amygdalopiriform	ND	+++	+
Entorhinal	ND	+++	+
Basal forebrain
Anterior olfactory n	++	+++	+++
Dorsal endopiriform n	+	++	+
Olfactory tubercle	tr	+	+
(Accumbens rostal pole)	+	++	++
(Accumbens core)	+	++	++
(Accumbens shell)	+	++	++
Islands of Calleja	tr	+	+
(Lateral septum, i)	+	+	+
(Lateral septum, v)	+	++	++
m septum-diagonal band	+	+	++
(Caudate-putamen)	tr	++	+
(entral pallidum)	tr	+	+
(dl bed n stria terminalis)	+	++	+
(dm bed n stria terminalis)	+	+	++
(v bed n stria terminal)	+	++	++
(Globus pallidus)	0	tr	tr
(Central n amygdala, l)	+	+	++
(Central n amygdala, m)	tr	+	++
Basal n amygdala	+	++	++
Medial n amygdala	+	++	++
Preoptic area and lateral hypothalamus
Lateral preoptic area	+	++	++
Medial preoptic area	+	++	++
(Suprachiasmatic n)	+++	+++	+++
(Supraoptic n)	+	++	+
Lateral hypothalamus	++	++	++
Medial hypothalamus	+	++	++
(Paraventricular n)	+	+	+
Dorsomedial n	+	++	++
Ventromedial n	+	+	+
Posterior n	+	++	+
Supramammillary n	++	++	+
Mammillary n	+	+	+
Thalamus, epithalamus, subthalamus
Parataenial	+	+	+
Midline-intralaminar	++	+++	+++
Reuniens	+	++	+
Anterodorsal	+	++	++
Mediodorsal	tr	+	+
Reticular	0	0	0
Ventral anterior	+	+	+
Interanteromedial	++	++	++
Ventromedial	+	+	+
Ventrolateral	+	+	+
Ventral posterolateral	+	tr	+
Ventral posteromedial	+	tr	+
(l Habenula, l)	0	tr	tr
(l Habenula, m)	tr	++	++
Lateral geniculate	+	++	++
Entopeduncular n	tr	tr	tr
(Subthalamic n)	tr	+	tr
(Zona incerta)	++	+	+
Midbrain and pons
(Ventral tegmental area)	tr	tr	tr
Substantia nigra compacta	tr	tr	tr
(Subst nigra reticulata)	tr	tr	tr
Medial geniculate n	++	++	++
(Rostromed tegmental n)	0	0	0
Periaqueductal gray	+	++	++
Sup colliculus, superficial	+	++	+
Sup colliculus, intermed	+	++	+
Sup colliculus, deep	+	++	+
Pontine n	+++	+++	+++
Deep mesencephalic n	+	+	+
Red n	tr	+	+
Interpeducnular n	tr	+	+
Retrorubral field	tr	+	+
(Pedunculopontine teg n)	tr	+	+
Dorsal raphe n	++	+	+
Median raphe n	+	+	+
Cuneiform n	+	+	+
Inferior colliculus	+++	+++	+++
Lateral lemniscus	+	+	+
Pont retic n, oral	+	+	+
(Laterodorsal tegmental n)	+	+	+
Ventral tegmental n	++	++	++
Dorsal tegmental n	++	++	++
Parabrachial n	+	+	++
Trigeminal n, motor	tr	tr	+
Trigeminal n, sensory	tr	+	+
Superior olivary n	0	+	+
Locus ceruleus	0	+	+
Medulla
Caud pontine reticular n	tr	+	+
Facial n	tr	tr	tr
Abducens n	0	tr	tr
Medial vestibular n	tr	+	+
Cochlear n	+	++	+
Trigeminal n, sensory	tr	tr	+
Gigantocellular retic n	tr	+	+
Lateral reticular n	tr	+	+
n Prepositus	tr	+	+
n Tracts solitarius	tr	+	+
n Ambiguous	tr	+	+
Dorsal motor n vagus	tr	tr	+
Hypoglossal n	tr	tr	+
Medullary dorsal horn	tr	+	++
Cerebellum
Granule cells	+++	+++	+++
Deep nn	tr	tr	tr

^aTable entries reflect the averages of three cases, which were computed by assigning observations of 0, 0–10, 11–75, 76–200, and more than 200 Fos-ir nuclei/20x field numerical values of 0, 0.5, 1, 2, and 3. Averages of 0 and in the ranges of 0.01–0.66, 0.67–1.44, 1.45–2.22, and 2.23–3 are listed in the table as 0, tr (trace), +, ++, and +++, respectively. Entries in parentheses were subjected to additional objective analysis illustrated in Figures 5, 6, 7 and 8 and Table 2.

dl,dorsolateral; dm,dorsomedial; I,intermediate; ir,immunoreactive; l,lateral; m- medial; n,nucleus; ND,not determined; nn,nuclei; retic,reticularis; rostromed,rostromedial; subst,substantia; sup,superior; teg,tegmental; v,ventral.