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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Immunol. 2010;260(2):119–127. doi: 10.1016/j.cellimm.2009.10.002

Figure 5.

Figure 5

Absolute numbers of CD4+ T cells in the lymph nodes and spleen of adult d3tx A/J and B6 mice. The total number of CD4+ T cells isolated from the (A) Lymph nodes and (B) spleen of natural and cross-fostered B6 and A/J mice were analyzed by FACS. CD4+ T cells from the lymph nodes of cross-fostered A/J mice are significantly lower than naturally nursed A/J mice. The significance of differences was determined using the unpaired Student's t-test (p < 0.02). The spleen from A/J mice naturally exhibit reduced numbers of CD4+ T cells compared to B6 that is independent of maternal influence. The significance of differences was determined using one way ANOVA followed by Bonferroni's multiple comparison test (p = 0.0002). B6-B6, B6 mother and B6 pup; B6-A/J, B6 mother and A/J pup; A/J-B6, A/J mother and B6 pup; A/J-A/J, A/J mother and A/J pup.