Fig. 7.

Nuclear translocation of NFκB is involved in the LPS-induced increase of CHL1 expression in cultured astrocytes. A. Cells preincubated with the indicated concentrations of IKK-NBD peptide for 1 h were stimulated with 1.0 μg/ml LPS for 3 h. Cell lysates were subjected to immunoblot analysis of CHL1 expression. B–C. Equal amounts of proteins from control and stimulated cells were subjected to immunoblot analysis, and phosphorylation of NFκB isoforms was determined in the cytosolic and nuclear fractions. D. Cells were preincubated with Rottlerin (50 nM), LY294002 (10 μM), and PD98059 (20 μM) for 1 h before LPS treatment for 3 h. Phosphorylation of p65 NFκB in the nuclear fraction was subjected to immunoblot analysis.