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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Cell Signal. 2010 Mar;22(3):366–376. doi: 10.1016/j.cellsig.2009.10.005

Figure 8. Giα1 inhibits Raf kinase binding to RGS14.

Figure 8

HeLa cells were transfected with HA-Raf-1 alone or in the presence of either Flag-RGS14, Flag-RGS14 plus Giα1-EE, RGS14 plus HA-H-Ras(GV) or Flag-RGS14 plus H-Ras(GV) and Giα1-EE. Cells were transfected as indicated by the labels above the lanes in the gel. Cell extracts were prepared and subjected to anti-Flag immunoprecipitation (IP), and recovered proteins were detected by immunoblot analysis using anti-HA (H-Ras and Raf-1), anti-EE (Giα1) or anti-Flag (RGS14). The cell lysates shown in the lanes represent 5% of the whole cell extracts used for IP. Results are representative of at least three separate experiments for each condition.