Table 2.
Increased HLA-A*0201 host peptides following influenza A infection
| Fractiona | Ionb | Peptide | Protein | AAc | IC50d | PR8 | 7485 | 309 | Gene |
|---|---|---|---|---|---|---|---|---|---|
| 45 | 460.2 | FAGDDAPRA | Actin | 9 | 1083.8 | NA | 5.21 | 7.48 | ACTB |
| 45 | 500.2 | NLAEKPKTV | Proteasome associated protein ECM29 | 9 | 281.2 | NA | 2.84 | 2.66 | ECM29 |
| 46 | 508.2 | ALKDKIEKA | Heat shock protein 94c | 9 | 1392.9 | 2.8 | 3.2 | NA | GRP94C |
| 46 | 800.4 | IIPASTGAA | Glyceraldehyde-3 phosphate dehydrogenase | 9 | 6817.7 | 17.7 | 12.67 | NA | GAPDH |
| 47 | 450.2 | IINEPTAAA | Heat shock protein 70 | 9 | 1060.7 | 21.71 | 4.28 | NA | HSPA2/5/6/8/1A/1B/1L |
| 48 | 546.2 | GVYDGEEHSV | Melanoma antigen, family B | 10 | 289 | 9.92 | 1.97 | 1.66 | MAGEB2 |
| 50 | 464.7 | VVQDGIVKA | Peroxiredoxin 5 | 9 | 880.2 | 7.38 | 10.11 | NA | PRDX5 |
| 53 | 516.2 | GLIKGSGTAEV | Pyruvate kinase | 11 | 1940.7 | NA | 2.23 | 1.69 | PKM2 |
| 53 | 537.2 | KLDQDLNEV | Structural maintenance of chomosome 3 | 9 | 22.5 | 1.98 | 2.76 | 1.57 | SMC3 |
| 53 | 578.8 | FIQTQQLHAA | Pyruvate kinase | 10 | 2280 | 10.17 | 24.26 | NA | PKM2 |
| 53 | 959.5 | SQVPEVTTV | Phosphoinositide-3-kinase, regulatory subunit 4 | 9 | 335.2 | NA | 7.52 | 1.55 | PIK3R4 |
| 54 | 497.2 | NLDPAVHEV | GTPase 1 | 9 | 27.8 | 5.03 | 2.88 | NA | GPN1 |
| 54 | 499.8 | RVPPPPPIA | Heterogeneous nuclear ribonucleoprotein C | 9 | 8604 | 6.54 | 4.96 | NA | HNRNPC |
| 54 | 502.7 | NMVAKVDEV | Ribosomal protein L10a | 9 | 116.9 | 2.93 | 2.12 | NA | RPL10A |
| 54 | 566.7 | TLYEHNNEL | Achalasia, adrenocortical insufficiency, alacrimia | 9 | 81.5 | 2.49 | 2.84 | 2.2 | AAAS |
| 55 | 589.7 | YAYDGKDYIA | MHC-B antigen | 10 | 413.9 | 1.6 | 2.74 | NA | HLA-B |
| 56 | 444.2 | AVSDGVIKV | Cofilin 1 | 9 | 140.4 | 8.98 | 2.02 | NA | CFL1 |
| 56 | 530.2 | ILGYTEHQV | Glyceraldehyde-3 phosphate dehydrogenase | 9 | 171.1 | 23.91 | 4.47 | NA | GAPDH |
| 57 | 495.3 | TLAEVSTRL | Salt-inducible protein kinase-like kinase | 9 | 101.6 | NA | 1.88 | 2.61 | SNF1LK |
| 58 | 432.2 | LLDVVHPA | TCP-1 HIV-1 Nef interacting protein | 8 | 644.4 | 33.09 | 1.83 | 1.7 | CCT7 |
| 58 | 467.7 | LLDVVHPAA | TCP-1 HIV-1 Nef interacting protein | 9 | 265 | 3.43 | 3.82 | NA | CCT7 |
| 60 | 474.2 | ILSGVVTKM | Ribosomal protein S11 | 9 | 384.7 | 1.95 | 1.59 | NA | RPS11 |
| 60 | 567.3 | ILMEHIHKL | Ribosomal protein L19 | 9 | 9.8 | 5.16 | 3.04 | NA | RPL19 |
| 62 | 507.3 | ALNEKLVNL | Eukaryotic translation initiation factor 3, subunit F | 9 | 110.7 | 1.52 | 2.49 | NA | EIF3F |
| 62 | 528.7 | ALNDHFVKL | Glyceraldehyde-3 phosphate dehydrogenase | 9 | 95.4 | 23.02 | 8.32 | NA | GAPDH |
| 64 | 455.8 | GVLPNIQAV | Histone H2A | 9 | 509.1 | NA | 8.69 | 4.16 | HIST3H2A |
| 64 | 466.7 | ALDKATVLL | Programmed cell death 4 | 9 | 100.7 | 2.13 | 3.8 | NA | PDCD4 |
| 64 | 514.8 | GLIEKNIEL | DNA methyltransferase | 9 | 56.7 | 1.58 | 1.54 | NA | DNMT1 |
| 64 | 528.8 | KVFDPVPVGV | Deadbox polypeptide 9 | 10 | 112.8 | 1.74 | 1.97 | NA | DHX9 |
| 64 | 630.3 | TLWDIQKDLK | Lactate dehydrogenase B | 10 | 17930.7 | 1.64 | 4.79 | NA | LDHB |
| 65 | 1014.5 | SMTLAIHEI | Degenerative spermatocyte homolog 1 | 9 | 143.6 | 2.11 | 1.58 | NA | DEGS1 |
| 66 | 471.2 | GLDIPTVQV | Deadbox polypeptide 49 | 9 | 193.4 | 4.78 | 1.78 | NA | DDX49 |
| 66 | 479.2 | GLIDGRLTI | Signal peptidase complex subunit 2 | 9 | 201.1 | 1.67 | 2.21 | NA | SPCS2 |
| 67 | 544.2 | TLWGIQKEL | Lactate dehydrogenase A | 9 | 197.4 | 3.27 | 1.72 | NA | LDHA |
| 67 | 878.5 | AIIGGTFTV | ER-golgi intermediate compartment 1 | 9 | 27 | 4.17 | 2.79 | NA | ERGIC |
| 68 | 476.2 | LLGPRLVLA | Transmembrane trafficking protein 10 | 9 | 276.8 | 2.09 | 5.6 | NA | TMED10 |
| 68 | 480.2 | SLLEKSLGL | Eukaryotic translation elongation factor 1 | 9 | 46 | 1.64 | 3.7 | NA | EEF1E1 |
| 68 | 622.8 | KLIGDPNLEFV | Ras-related nuclear protein | 11 | 245.3 | 2.82 | 7.2 | NA | RAN |
| 69 | 537.2 | IMLEALERV | Small nuclear ribonucleoprotein polypeptide G | 9 | 14.1 | 1.64 | 1.54 | NA | SNRPG |
| 70 | 537.7 | SMPDFDLHL | AHNAK nucleoprotein | 9 | 85.4 | 1.83 | 2.78 | NA | AHNAK |
| 70 | 625.8 | FLAEEGFYKF | Subunit of the oligosaccharyltransferase complex | 10 | 284.3 | 2.98 | 2.86 | NA | STT3A |
| 70 | 1019.5 | GIVEGLMTTV | Glyceraldehyde-3 phosphate dehydrogenase | 10 | 205.5 | 3.1 | 1.57 | NA | GAPDH |
| 73 | 541.3 | TLAKYLMEL | Cyclin B1 | 9 | 16.4 | 6.81 | 3.95 | NA | CCNB1 |
| 73 | 588.3 | GLQDFDLLRV | Protein kinase C, iota | 10 | 43.7 | 2.29 | 2.9 | NA | PRKCI |
| 74 | 485.8 | LLDVTPLSL | Heat shock protein 70 | 9 | 153.6 | 9.68 | 1.92 | NA | HSPA2/8/9 |
| 75 | 488.7 | GIWGFIKGV | Proline-rich coiled-coil 1 | 9 | 116.7 | 6.1 | 2.01 | NA | PRRC1 |
| 75 | 533.8 | FLPSYIIDV | Cleavage and polyadenlyation specificity factor 1 | 9 | 2.8 | 2.57 | 2.97 | NA | CPSF1 |
| 75 | 593.8 | IIMLEALERV | Small nuclear ribonucleoprotein polypeptide G | 10 | 43.2 | 13.68 | 4.89 | NA | SNRPG |
| 76 | 515.3 | SLLDIIEKV | Tuberous sclerosis 2 | 9 | 9.8 | 2.56 | 1.82 | NA | TSC2 |
| 79 | 558.2 | SLFEGTWYL | Hydroxymethylglutaryl coA synthase | 9 | 2.6 | 2.36 | 1.83 | 2.79 | HMGCS1 |
a
RP-HPLC fraction containing the endogenous peptide
b
Ion mass in atomic mass units corresponding to the endogenous peptide
c
Amino acids
d
IC50=Inhibitory concentration 50 percent