Table 2.
Singleton modifications and vector nomenclature per clade.
| Clade | Parental | R1/R | R2 | R3 | R4 | R5 | .1 | .2 |
|---|---|---|---|---|---|---|---|---|
| A | hu.44 | G609D | P446L | E137K | ||||
| hu.46 | P156S | R362C | S393F | A676 | ||||
| hu.48 | G277S | E322K | S552N | |||||
| AAV6 | K531E | F129L | ||||||
| B | hu.29 | G396E | ||||||
| D | rh.48 | S304N | K217E | |||||
| rh.67 | L119F | |||||||
| rh.37 | E634K | T207M | ||||||
| cy.5 | G13D | D403N | R51K | N158K | P161Q | |||
| E | rh.64 | R697W | V686E | |||||
| rh.58 | S653N | C696R | ||||||
| rh.2 | V651I | |||||||
| Other | ch.5 | T611I | ||||||
| rh.8 | D531E | |||||||
| rh.32 | S354P | G409E | Y419H | P423L | L482F |
Natural AAV isolates identified per clade A–E or without clade affiliation in 'Other'10,20 side-by-side with singleton changed capsid variants. The suffix R refers to the number of singleton residues that were changed. For these changes, the last sequential reversion is described by the location (wild type sequence VP1 numbering) and residue change. For selected isolates, singleton changes were done individually (as opposed to sequentially) and denoted with ".1" and ".2". When both those individual non-conservative mutations were combined, they yielded an R2 clone.