Table 3.

Impact of singleton residues on vector yields.

Clade	Isolate	Parental	R1/R	R2	R3	R4	R5	.1	.2
A	AAV1	141
	hu.44	0.42	0.49	4	87
	hu.46	<0.04	<0.04	<0.04	<0.04	0.11
	hu.48	0.30	0.38	99	148
	AAV6	38		110				118	42
B	AAV2	25
	hu.29	0.07	110
	hu.51	27
	hu.13	118
C	hu.11	68
	hu.53	33
D	AAV7	141
	rh.48	1.86		76				13	19
	rh.67	1.75	2.01
	rh.37	1.03		84				57	72
	cy.5	103	106	103	99		87
E	AAV8	129
	rh.39	125
	rh.10	80
	hu.37	144
	rh.43	99
	rh.64	0.19	72	125
	rh.46	28
	rh.58	3.57		0.42
	rh.20	61
	rh.2	19	29
	pi.2	22
F	AAV9	133
	hu.32	182
Other	rh.8	80	120
	rh.32.33	0.49					210
	ch.5	57	33

Numbers represent average GC particles per producer cell (gc/cell) × 10³) quantified by Taqman PCR (limit of detection: 0.04 gc/cell) for all of the wild type and capsid modified AAV vectors. Natural AAV isolates segregated per clade A–E or as other when no clade affiliation is available¹⁰,²⁰ presented with singleton changed capsid variants. The suffix R refers to the number of sequentially changed singleton residues. For selected isolates, singleton changes were done individually (as opposed to sequentially in a cumulative fashion) and denoted with ".1" and ".2".