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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2009 Dec;8(12):3223–3233. doi: 10.1158/1535-7163.MCT-09-0794

Fig. 3.

Fig. 3

c-Myc is induced by arginine-depletion and positively regulates AS promoter activity through the E-box in A2058 cells. A. Cells were transfected with 100 nM of scramble (contol) or c-Myc siRNA. Cells were maintained in normal, ADI-containing, or arginine-free medium for 48 h. Expression levels of AS, c-Myc and β-actin were analyzed by Western blotting. B. Cells were transfected with control or Sp4 siRNA and maintained in normal medium, medium containing 0.05 µg/ml ADI or arginine-free medium for 72 h. Expression levels of AS, Sp4 and β-actin were analyzed by western blotting. C. The interaction between c-Myc and Sp4 was analyzed by co-immunoprecipitation using anti-c-Myc antibody followed by Western blotting using anti-Sp4 and anti-c-Myc antibodies. D. The interaction between Sp4 and c-Myc was analyzed by co-immunoprecipitation using anti-Sp4 antibody followed by Western blotting using anti-c-Myc and anti-Sp4 antibodies.