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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Int J Cancer. 2010 Jan 15;126(2):533–544. doi: 10.1002/ijc.24725

FIGURE 2.

FIGURE 2

Subcellular localization of rhodamine labeled Bac-ELP1-p21 in SKOV-3 cells as visualized by confocal microscopy. Cells were treated with 20 µM of rhodamine-labeled Bac-ELP1-p21 at 37°C or 42°C for 1 h. Confocal images were taken (A) 1 h and (B) 24 h later. Tubulin was stained as a reference for cellular structure. The subcellular distribution of Bac-ELP1-p21 was also confirmed in live cells 24 h after a 1 h exposure at 37 or 42 °C (C). Fluorescence intensity in the heated samples was greater and the gain was adjusted for these samples before image acquisition. Therefore, these images are not quantitative.