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The comparison of optimized method with explants and enzymatic digestion method
| Time required for primary culture | Contamination by other cells | Special equipment or reagent | |
|---|---|---|---|
| Optimized method | 8 days | Little | 0.25% Trypsin |
| Explant method | Several weeks | EC, Fb | No |
| Enzymatic method | Within 1 day but takes 3–6 Days for cells to reach confluence |
Little | Trypsin, collagenase, soybean trypsin inhibitor |
EC indicates endothelial cells; Fb indicates fibroblasts
