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. 2009 Dec 29;4(12):e8240. doi: 10.1371/journal.pone.0008240

Figure 2. Growth curves of the EHOS E4 isolate and its corresponding irp2 knockout strain.

Figure 2

The results are presented as the mean of three experiments performed in duplicate on different days. Cells were grown in: A) nutrient broth treated with 1% Chelex; B) nutrient broth treated with 1% Chelex and supplemented with 80% iron-saturated lactoferrin (∼6.2 µM, Fe-LF) or unsaturated lactoferrin (∼6.4 µM,0-LF); C) nutrient broth treated with 1% Chelex and supplemented with 17 µM holo-transferrin or 17 µM apo-transferrin; D) nutrient broth treated with 1% Chelex and supplemented with 10 µM or 30 µM hemin.