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. 2009 Dec 24;4(12):e8457. doi: 10.1371/journal.pone.0008457

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Wallner et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Bet v 1.0401 batch A was purified from E. coli lysates using a denaturing step during production, batch B was purified from soluble bacterial lysate. Production batch A, lane 1, total bacterial lysate; lane 2, soluble bacterial lysate; lane 3, fraction after basic denaturation; lane 4, salt precipitated fraction; lane 5 hydrophobic interaction chromatography; lane 6, reversed phase chromatography. Production batch B, lane 1, total bacterial lysate; lane 2, soluble bacterial lysate; lane 3, salt precipitated pellet; lane 4, salt precipitated fraction; lane 5, hydrophobic interaction chromatography; lane 6, anion exchange chromatography.