Table 1. Recombinant production of Bet v 1.0401.

	Production batch A	Production batch B
Gene	Bet v 1.0401, plant codon usage	Bet v 1.0401, E. coli codon usage
Expression plasmid, cloning sites	pET28b, 5′ Nco I, 3′ Eco RI	pET28b, 5′ Nco I, 3′ Eco RI
Bacterial cells	BL21 (DE3)	BL21 Star™ (DE3)
Expression conditions	37°C, 6 h	16°C, 18 h
Extraction	inclusion bodies with NaOH, pH 13	soluble proteins with 0.5 M Urea
Chromatography	HIC, reversed phase HPLC	HIC, IEX