Table 3.
Invitrogen SelectScreen Kinase Profiling data for apigenin, genistein, quercetin and rutin (% inhibition ± SEM at 50 µM)
| Apigenin | Genistein | Quercetin | Rutin | |
|---|---|---|---|---|
| Fyn | 54.5 ± 4.1 | 16.4 ± 2.2 | 75.4 ± 0.7 | 0.7 ± 0.5 |
| Lyn A | 53.9 ± 0.8 | 20.4 ± 0.6 | 54.0 ± 1.9 | 0.8 ± 0.6 |
| Src | 52.1 ± 1.4 | 19.3 ± 1.1 | 63.9 ± 2.2 | 13.7 ± 0.8 |
| Syk | 68.2 ± 5.3 | 18.6 ± 2.1 | 80.4 ± 0.1 | 8.5 ± 0.2 |
| MAPK1 (ERK2) | 0.9 ± 0.6 | 10.1 ± 2.7 | 20.7 ± 0.5 | 0.0 ± 0.0 |
| MAPK3 (ERK1) | 0.0 ± 0.0 | 5.9 ± 1.0 | 9.3 ± 1.4 | 0.0 ± 0.0 |
| MAPK14 (p38α) | 16.1 ± 3.7 | 16.8 ± 0.4 | 16.0 ± 1.4 | 0.0 ± 0.0 |
| AKT1 (PKBα) | 13.8 ± 1.6 | 16.1 ± 2.0 | 46.2 ± 1.0 | 0.0 ± 0.0 |
| AKT2 (PKBβ) | 13.9 ± 4.1 | 13.1 ± 1.3 | 51.2 ± 5.0 | 0.6 ± 0.5 |
| PRKCA (PKCα) | 0.0 ± 0.0 | 0.0 ± 0.0 | 9.8 ± 7.1 | 2.7 ± 1.9 |
| PRKCB1 (PKCβ1) | 10.7 ± 0.7 | 5.9 ± 0.9 | 24.0 ± 1.9 | 1.4 ± 1.0 |
| PRKCD (PKCδ) | 0.0 ± 0.0 | 2.7 ± 1.9 | 23.2 ± 2.3 | 0.0 ± 0.0 |
| PI3Kβ | 32.0 ± 2.8 | 4.0 ± 0.7 | 75.5 ± 2.5 | 12.5 ± 0.4 |
| PI3Kγ | 21.0 ± 2.1 | 13.5 ± 0.4 | 85.5 ± 1.8 | 37.0 ± 2.1 |
| PI3Kδ | 25.5 ± 3.2 | 7.5 ± 2.5 | 99.0 ± 0.7 | 99.0 ± 0.0 |
The activity of several flavonoids against a panel of protein kinases was assessed using a fluorescence resonance energy transfer method. The assays were performed in 1% dimethylsulphoxide and an ATP concentration equal to the Km[app] of each kinase. MAPK, mitogen-activated protein kinase; PKB, protein kinase B; PRKC, protein kinase C; PI3-kinase, phosphatidylinositol 3-kinase. Data were obtained from two different experiments.