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Time-dependent effects of isoprenaline alone (Iso, 10 µM) and in the additional presence of carbachol (Carb) on current through the L-type Ca2+-channels (ICa, nA) in isolated, patch-clamped, ventricular cardiomyocytes from littermate wild-type mice (Gi2α+/+, A) compared with Gi2α knockout mice (Gi2α−/−, B). Representative tracings from five cardiomyocytes from three different Gi2α+/+ and Gi2α−/− hearts are shown.
