Table 2.
Binding and functional data from cells expressing NMU1 or NMU2
| Radioligand | Cell type | Receptor | KD nmol·L−1 | Assay | Ligand | EC50 nmol·L−1 | |
|---|---|---|---|---|---|---|---|
| Howard et al. (2000) | [125I]-NMU-23 (rat) | HEK-293 | hNMU1 | 0.3 | ↑[Ca2+]i | NMU-25 (human) | 1.0 |
| NMU-23 (rat) | 0.75 | ||||||
| NMU-8 (pig) | 0.11 | ||||||
| NMU-25 (pig) | 1.36 | ||||||
| hNMU2 | ↑[Ca2+]i | NMU-25 (human) | 1.0 | ||||
| NMU-23 (rat) | 2.9 | ||||||
| NMU-8 (pig) | 0.3 | ||||||
| NMU-25 (pig) | 2.8 | ||||||
| Fujii et al. (2000) | [125I]-NMU-8 (pig) | CHO | hNMU1 | 0.066 | ↑[Ca2+]i | NMU-23 (rat) | 1.3 |
| Hosoya et al. (2000) | [125I]-NMU-8 (pig) | CHO | hNMU2 | 0.022 | ↑AA release | NMU-25 (human) | 4.0 |
| NMU-23 (rat) | |||||||
| NMU-25 (pig) | 1.4–2.0 | ||||||
| NMU-8 (pig) | |||||||
| Raddatz et al. (2000) | [125I]-NMU-23 (rat) | COS-7 | hNMU1 | 0.61 | ↑[Ca2+]i | NMU-25 (human) | 4.0 |
| NMU-23 (rat) | 2.1 | ||||||
| NMU-25 (pig) | 5.2 | ||||||
| NMU-8 (pig) | 1.1 | ||||||
| hNMU2 | 0.81 | NMU-25 (human) | 2.4 | ||||
| NMU-23 (rat) | 5.0 | ||||||
| NMU-25 (pig) | 3.0 | ||||||
| NMU-8 (pig) | 1.2 | ||||||
| 125I]-NMU-8 (pig) | hNMU1 | 1.2 | |||||
| hNMU2 | 0.83 | ||||||
| Szekeres et al. (2000) | HEK-293 | hNMU1 | ↑[Ca2+]i | NMU-25 (pig) | 0.38 | ||
| NMU-8 (pig) | 0.21 | ||||||
| NMU-23 (rat) | 0.17 | ||||||
| ↑IP | NMU-25 (pig) | 0.28 | |||||
| Shan et al. (2000) | HEK-293 | hNMU2 | ↑[Ca2+]i | NMU-25 (human) | 5 | ||
| Hedrick et al. (2000) | HEK-293 | hNMU1 | ↑[Ca2+]i | NMU-25 (human) | 12 | ||
| NMU-8 (pig) | 10 | ||||||
| Kojima et al. (2000) | CHO | hNMU1 | ↑[Ca2+]i | NMU-25 (human) | 0.25 | ||
| NMU-23 (rat) | 7.0 | ||||||
| NMU-25 (pig) | 0.8 | ||||||
| NMU-8 (pig) | 2.8 | ||||||
| Funes et al. (2002) | HEK-293 | mNMU1 | ↑[Ca2+]i | NMU-25 (human) | 20 | ||
| NMU-23 (mouse) | 8.8 | ||||||
| NMU-8 (pig) | 9.5 | ||||||
| mNMU2 | NMU-25 (human) | 2.2 | |||||
| NMU-23 (mouse) | 1.6 | ||||||
| NMU-8 (pig) | 3 | ||||||
| Brighton et al. (2004b) | [125I]-NMU-25 (human) | HEK-293 | hNMU1 | 0.14 | ↑[Ca2+]i | NMU-25 (human) | 0.72 |
| ↑IP | 0.39 | ||||||
| ↓cAMP | 0.08 | ||||||
| hNMU2 | 0.11 | ↑[Ca2+]i | 1.07 | ||||
| ↑IP | 0.43 | ||||||
| ↓cAMP | 0.09 | ||||||
| Johnson et al. (2004) | [125I]-NMU-25 (human) | T-cell clone | mNMU1 | 0.36 | ↑[Ca2+]i | NMU-25 (human) | 4.8 |
| NMU-23 (rat) | 10.1 | ||||||
| NMU-25 (pig) | 6.0 | ||||||
| NMU-8 (pig) | 4.8 | ||||||
| Aiyar et al. (2004) | [125I]-NMU-25 (human) | HEK-293 | hNMU1 | 0.08 | ↑IP | NMU-25 (human) | 1.6 |
| hNMU2 | 0.16 | 1.5 | |||||
| hNMU1 | ↑[Ca2+]i | 0.50 | |||||
| hNMU2 | 0.50 | ||||||
| Garlton et al. (2004) | HEK-293 | hNMU1 | ↑[Ca2+]i | NMU-23 (rat) | 1.25 | ||
| hNMU2 | 1.10 | ||||||
| Shukla et al. (2007) | [125I]-NMU-23 (rat) | Yeast | hNMU2 | 0.79 | |||
| BHK | 0.96 | ||||||
| Xia et al. (2008) | [125I]-NMU-23 (rat) | BHK | hNMU1 | 0.14 |
‘h’ in front of NMU1 or NMU2 denotes human and ‘m’ murine.
AA, arachidonic acid; BHK, baby hamster kidney; cAMP, cyclic adenosine monophosphate; CHO, Chinese hamster ovary; HEK, human embryonic kidney; IP, inositol phosphates; NMU, neuromedin U.