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. 2009 Apr 27;158(1):328–338. doi: 10.1111/j.1476-5381.2009.00182.x

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Journal compilation © 2009 The British Pharmacological Society

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Characterization of [³H]-SB265610 in CHO membranes expressing human CXCR2 receptor. (A) [³H]-SB265610 saturation binding isotherm to determine K_d and B_max. (B) Dissociation curve for [³H]-SB265610 to determine the k_off. (C) A family of [³H]-SB265610 association curves for the determination of k_ob Graphs (A), (B) and (C) are representative of ≥3 separate experiments. (D) A plot of k_ob against concentration of [³H]-SB265610, the data shown are the individual k_ob from three separate experiments. The slope of the line represents the k_on for [³H]-SB265610 and the y-intercept the k_off. CHO, chinese hamster ovary; CXCR, C-X-C chemokine receptor.