Figure 7.

Stimulation of [³⁵S]-GTPγS binding in CHO-CXCR2 membranes by IL-8 (A) and GROα (B) in the absence and presence of a range of concentrations of SB265610 (10, 100 and 1000 nmol·L⁻¹). The data were globally analysed using the operational allosteric ternary complex model (Equation 6). The best-fit parameter values for the IL-8 data were: K_B=−8.71, K_A=−6.02 and τ= 147.5, and for the GROα data; K_B=−8.66, K_A=−6.34 and τ= 239.5. Data shown are representative of three separate experiments, performed in duplicate. CHO, chinese hamster ovary; CXCR, C-X-C chemokine receptor; GRO, growth related oncogene; IL-8, interleukin-8.