FIG. 6.

Consequences of replacements in BioN for complex formation and ATPase activity. BioMNY complexes were purified using the His tag on BioM. Two micrograms of protein was subjected to SDS-PAGE, and the gel was stained with Coomassie blue. The molecular masses of the standard proteins are 25, 20, and 15 kDa. The number below each lane represents the ATPase activity of the purified sample, expressed in nmol P_i min⁻¹ (mg protein)⁻¹. The BioM_K42NNY complex (left lane) with inactivated ATPase domains served as a negative control.