FIG. 6.

RT-PCR analysis of the pra gene cluster in JJ-1b. Total RNA used for cDNA synthesis was isolated from JJ-1b cells grown on 4HB (A), PCA (B), and succinate (C). Agarose gel electrophoresis of RT-PCR assays with primers targeting praR (expected size, 572 bp), praR-praA (expected size, 1,092 bp), praA-praE (expected size, 2,190 bp), praE-praF (expected size, 1,940 bp), praF-praC (expected size, 1,553 bp), and praC-praI (expected size, 1,779 bp) are shown. Positions of primer pairs and primer sequences are indicated in Fig. 1B and in Table S1 in the supplemental material, respectively. Lane M, molecular weight markers; lanes + and −, RT-PCR with and without reverse transcriptase, respectively; lanes G, control PCR with the JJ-1b genomic DNA. RT-PCR of the 16S rRNA gene was used as a control to confirm equivalent quantities of template loading.