TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| Paenibacillus sp. strain JJ-1b | Wild type (ATCC 35889) | 9 |
| E. coli | ||
| DH5 | supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 21 |
| DH5α | Δ(lac)U169 φ80 Δ(lacZ)M15 hsdR17 endA1 gyrA96 recA1 relA1 supE44 thi-1 | 21 |
| BL21(DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3); T7 RNA polymerase gene under the control of the lacUV5 promoter | 59 |
| Plasmids | ||
| Charomid 9-36 | Apr cos | 50 |
| pBluescript II KS(+) | Cloning vector; Apr | 56 |
| pUC19 | Cloning vector; Apr | 65 |
| pT7Blue | Cloning vector; Apr T7 promoter | Novagen |
| pET21a(+) | Expression vector; Apr T7 promoter | Novagen |
| pColdIV | Expression vector; AprcspA promoter | Takara Bio |
| pQF50 | Broad-host-range transcriptional fusion vector containing a promoterless lacZ; Apr | 17 |
| pPR9TT | Broad-host-range vector containing lacZ without ATG; Apr Cmr | 53 |
| pPR9TZ | pPR9TT with a 3.6-kb SmaI-ScaI fragment containing lacZ from pQF50 replacing the 3.2-kb BamHI fragment | K. Takamura |
| pTE2G1 | pT7Blue with a 563-bp PCR-amplified fragment containing praB internal region | This study |
| pCH221 | Charomid 9-36 with a 3.4-kb HindIII fragment carrying praA and praB | This study |
| pCE14 | Charomid 9-36 with a 1.5-kb EcoRI fragment | This study |
| pCK1 | Charomid 9-36 with a 4.0-kb KpnI fragment | This study |
| pCE3 | Charomid 9-36 with a 3.3-kb EcoRI fragment | This study |
| pCH1 | Charomid 9-36 with a 3.5-kb HindIII fragment | This study |
| pBH3 | KS(+) with a 3.4-kb HindIII fragment of pCH221 | This study |
| pBE1F | KS(+) with a 1.5-kb EcoRI fragment of pCE14 | This study |
| pBSK | KS(+) with a 1.4-kb SacI-KpnI fragment of pCK1 | This study |
| pBE3F | KS(+) with a 3.3-kb EcoRI fragment of pCE3 | This study |
| pBH4F | KS(+) with a 3.5-kb HindIII fragment of pCH1 | This study |
| pTINVF | pT7Blue with a 3.3-kb PCR fragment generated by the INVF and INVR primer pair | This study |
| pBINV2H2 | KS(+) with a 2.0-kb HindIII fragment of PCR fragment generated by the INV2F and INV2R primer pair | This study |
| pBBH | KS(+) with a 1.8-kb BamHI-HindIII fragment of pBH4F | This study |
| pBSS | KS(+) with a 0.3-kb SmaI-ScaI fragment of pBINV2H2 | This study |
| pTN23 | pT7Blue with a 603-bp PCR fragment generated by the praA-F and praA-R primer pair | This study |
| pTNB | pT7Blue with a 706-bp PCR fragment generated by the praB-F and praB-R primer pair | This study |
| pTCR | pT7Blue with a 392-bp PCR fragment generated by the praC-F and praC-R primer pair | This study |
| pTDF | pT7Blue with a 817-bp PCR fragment generated by the praD-F and praD-R primer pair | This study |
| pTH2 | pT7Blue with a 155-bp PCR fragment generated by the praH-F and praH-R primer pair | This study |
| pTIR | pT7Blue with a 617-bp PCR fragment generated by the praI-F and praI-R primer pair | This study |
| pETA23 | pET21a(+) with a 0.8-kb NdeI-EcoRI fragment carrying praA | This study |
| pETB23 | pET21a(+) with a 1.9-kb NdeI-HindIII fragment carrying praB | This study |
| pETC23 | pET21a(+) with a 0.4-kb NdeI-EcoRI fragment carrying praC | This study |
| pC4D23 | pColdIV with a 0.8-kb NdeI-XhoI fragment carrying praD | This study |
| pETH23 | pET21a(+) with a 1.9-kb NdeI-HindIII fragment carrying praH | This study |
| pETI23 | pET21a(+) with a 1.4-kb NdeI-BamHI fragment carrying praI | This study |
| pETCH23 | pET21a(+) with a 2.2-kb NdeI-HindIII fragment carrying praC and praH | This study |
| pBRI23 | KS(+) with a 9.4-kb SacI-ScaI fragment carrying pra genes | This study |
| pPZAR | pPR9TZ with a 1.3-kb SacI-SacII fragment carrying praR and praA promoter region | This study |
| pPZA | pPR9TZ with a 0.6-kb PstI-SacII fragment carrying the praA promoter region | This study |
a
Apr and Cmr, resistance to ampicillin and chloramphenicol, respectively.