TABLE 2.
Identification of pra gene functions
| Gene | Deduced molecular mass, Da (no. of amino acid residues) | Representative homolog | Identity (%)a | Accession no. |
|---|---|---|---|---|
| praR | 28,947 (258) | IclR-type transcriptional regulator from Bacillus licheniformis ATCC 14580 | 49 | AAU25433 |
| IclR-type transcriptional regulator (PcaU) from Acinetobacter baylyi ADP1 | 25 | AAC37157 | ||
| praA | 29,636 (266) | Hypothetical protein from B. licheniformis ATCC 14580 | 53 | AAU25429 |
| HPCA 2,3-dioxygenase (HpaD) from Pseudomonas sp. strain DJ-12 | 29 | AAL28115 | ||
| HPCA 2,3-dioxygenase (HpaD) from Burkholderia xenovorans LB400 | 28 | ABE33954 | ||
| HPCA 2,3-dioxygenase (HpcB) from Escherichia coli C | 26 | CAA38985 | ||
| praB | 53,188 (486) | Aldehyde dehydrogenase from B. licheniformis ATCC 14580 | 74 | AAU25432 |
| HMS dehydrogenase (NahI) from Pseudomonas putida G7 (NAH7) | 55 | BAE92168 | ||
| HMS dehydrogenase (XylG) from P. putida mt-2 (pWW0) | 54 | AAA26053 | ||
| praE | 27,687 (260) | HPD hydratase (BphH) from B. xenovorans LB400 | 52 | ABE37051 |
| HPD hydratase (NbaH) from Pseudomonas fluorescens KU-7 | 45 | BAC65306 | ||
| praG | 31,466 (294) | Acetaldehyde dehydrogenase (NahO) from Bacillus sp. strain JF8 | 61 | BAD08310 |
| Acetaldehyde dehydrogenase (NbaJ) from P. fluorescens KU-7 | 53 | BAC65307 | ||
| praF | 36,116 (337) | HOV aldolase (NahM) from Bacillus sp. strain JF8 | 58 | BAD08311 |
| HOV aldolase (XylK) from P. putida mt-2 (pWW0) | 51 | AAA25692 | ||
| praD | 28,125 (260) | Putative OCA decarboxylase from B. licheniformis ATCC 14580 | 64 | AAU25431 |
| OCA decarboxylase (XylI) from P. putida mt-2 (pWW0) | 46 | AAA25693 | ||
| praC | 7,012 (63) | Putative OCA tautomerase from B. licheniformis ATCC 14580 | 64 | AAU25430 |
| OCA tautomerase (DmpI) from Pseudomonas sp. strain CF600 | 49 | CAA43229 | ||
| praH | 35,570 (313) | Putative decarboxylase from B. licheniformis ATCC 14580 | 58 | AAU25434 |
| ACMS decarboxylase (NbaD) from P. fluorescens KU-7 | 24 | BAC65312 | ||
| praI | 44,262 (394) | Putative 4HB 3-hydroxylase from B. licheniformis ATCC 14580 | 66 | AAU25427 |
| 4HB 3-hydroxylase (PobA) from Arthrobacter sp. strain ATCC 51369 | 45 | BAE46572 |
a
Percent identity obtained by aligning the deduced amino acid sequences by use of the EMBOSS alignment tool.