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τ plots for the PexsC (A) and PexsD (B) promoters in the presence (open circles) and absence (closed circles) of ExsAHis. Values for τobs were calculated from abortive initiation assays measuring synthesis of the products pppGCUUU (PexsC) and pppAAAUU (PexsD). Calculated values for τobs were plotted on the ordinate as a function of reciprocal RNAP concentration.
