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Discovery of the new insertion sequence ISPpu21 within the srpS gene in P. putida S12 by PCR. (A) Locations of the PCR primers (1 through 4), sizes of the amplified regions, and insertion sites of ISS12 and ISPpu21. (B) The products resulting from the PCR were separated on a 0.7% (wt/vol) agarose gel. Lanes: 1, region amplified with primers 1 and 2; 2, region amplified with primers 3 and 4; M, molecular size markers.
