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Southern hybridization analysis of total DNA from P. putida S12 and P. putida S12TS. The DNA samples were digested with PstI (lanes 1 and 2) or EcoRI (lanes 3 and 4) and analyzed by Southern hybridization with a 352-bp DNA probe made from ISPpu21. Lanes 1 and 3 show the results for wild-type P. putida S12; lanes 2 and 4 show the results for P. putida S12TS.
