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. 2009 Aug 28;191(21):6665–6674. doi: 10.1128/JB.00344-09

FIG. 3.

FIG. 3.

A transposon insertion in glmS upregulates rraB. (A) Location of the transposon insertion in the glmS gene. glmS is cotranscribed with upstream glmU from the promoter glmUp1. (B) Growth curve of strains LZ001 (solid line) and LZ002 (glmS852::Tn5; broken line) cultured in M9 media with 0.2% glucose. (C) β-Galactosidase activities in strains LZ001 (white bars) and LZ002 (black bars) in the presence or absence of 20 μg/ml GlcN-6-P were measured in cells grown in M9 media and harvested at an OD600 of 0.2 to 0.3. (D) Complementation of the glmS852::Tn5 mutation with plasmid pBAD-glmS. E. coli strains LZ001 (white bars) and LZ002 (black bars) transformed with plasmid pBAD30 or pBAD30-glmS as indicated were grown overnight in M9 media with 0.2% glucose and subcultured into fresh M9 media with 0.2% glycerol. At an OD600 of 0.4 to 0.5, 0.2% arabinose was added. Cells were harvested 2 h after induction, and the β-galactosidase activity was determined.