TABLE 1.
Strains, plasmids and phage vectors
| Strain, plasmid, or phage vector | Description | Reference or source |
|---|---|---|
| BW21116 | Δlac-169 creC510 hsdR514 uidA(ΔMluI)::pir+ | 19 |
| CAG51025 | MC1061 φλ[rpoH P3::lacZ] ΔrseB nadB3140::Tn10 ΔdegS arg::Tn5 | 19 |
| CJ1825 | MC1061 (λez1) | 16 |
| DHB4 | (ara-leu)7697 araD139 ΔlacX74 galE galK rpsL phoR (phoA)PvuII malF3 thi/F lac-pro lacIq | 14 |
| EC-O | thi-1 relA1 Δ(pro-lac)X113 supE44/F42-114(FTs) lac | 49 |
| JCB570 | MC1000 phoR zih12::Tn10 | 3 |
| JCB571 | MC1000 phoR zih12::Tn10 dsbA::kan | 6 |
| KS474 | F−lacX74 galE galK thi rpsL(strR) ΔphoA(PvuII) degP41(ΔPstl)::ΩkanR | 50 |
| LZ001 | P90C λ (−152 to −1 nt)rraB-lacZ | This study |
| LZ002 | P90C λ (−152 to −1 nt)rraB-lacZ glmS852::Tn5 | This study |
| LZ003 | CJ1825 glmS852::Tn5 | This study |
| MC4100 | F−araD139 Δ(argF-lac) U169 flbB(flhD)5301 deoC1 ptsF(fruA)25 relA1 rbsR22 rpsL150 thiA | 2 |
| Mjf256.10 | DHB4 gshA::Kmr ΔtrxB::CmrahpC(V164G) | 15 |
| MZB001 | EC-O λ p0-lacZ | This study |
| MZB002 | EC-O λ (−239 to −1 nt)rraB-lacZ | This study |
| MZB003 | EC-O λ (−152 to −1 nt)rraB-lacZ | This study |
| MZB004 | EC-O λ (−239 to −153 nt)rraB-lacZ | This study |
| P90C | [ara Δ(lac-pro) thi] | 38 |
| pBAD30 | pACYA184 ori; Ampr pBAD | 18 |
| pBAD30-glmS | pACYA184 ori; Ampr, glmS under pBAD promoter | This study |
| pSP417 | pBR322 ori; Ampr, multiple cloning sites (8 restriction sites) upstream of a promoterless lacZ | 44 |
| pMZB002 | pSP417, (−239 to −1 nt)rraB-lacZ | This study |
| pMZB003 | pSP417, (−152 to −1 nt)rraB-lacZ | This study |
| pMZB004 | pSP417, (−239 to −153 nt)rraB-lacZ | This study |
| λRS45 | bla′-lacZsc imm21 ind22 | 49 |
| λRS74 | placUV5-lacZ imm21 ind22 | 49 |
| λMZB1 | Same as λRS74, but containing the p0-lacZ fusion | This study |
| λMZB3 | Same as λRS45, but containing the (−152 to −1 nt)rraB-lacZ fusion | This study |