TABLE 2.
Strains and plasmids
| Plasmid or strain | Relevant properties | Reference |
|---|---|---|
| Plasmids | ||
| pTrc99A | Expression vector for E. coli based on pKK233-2, carries a hybrid trp/lac promoter and the multiple cloning site of pUC18; Apr | 1 |
| pTrcspoIIIJ | Like pTrc99A, contains B. subtilis spoIIIJ | This study |
| pTrcspoIIIJHis | Like pTrc99A, contains B. subtilis spoIIIJ with His6 tag at 3′ end | This study |
| pTrcyqjG | Like pTrc99A, contains B. subtilis yqjG | This study |
| pTrcyqjGHis | Like pTrc99A, contains B. subtilis yqjG with His6 tag at 3′ end | This study |
| pTrcyidC | Like pTrc99A, contains E. coli yidC | This study |
| pNZ8901 | Expression vector for B. subtilis, mutated Pspa promoter; Cmr | 3 |
| pNZspoIIIJ | Like pNZ8901, contains B. subtilis spoIIIJ | This study |
| pNZspoIIIJHis | Like pNZ8901, contains B. subtilis spoIIIJ with His6 tag at 3′ end | This study |
| pNZyqjG | Like pNZ8901, contains B. subtilis yqjG | This study |
| pNZyqjGHis | Like pNZ8901, contains B. subtilis yqjG with His6 tag at 3′ end | This study |
| pET20batpE | pET20b (Novagen) containing E. coli atpE | 47 |
| pET20bBatpE | pET20b containing B. subtilis atpE | This study |
| E. coli strains | ||
| DH5α | supE44 hsdR14 recA1 endA1 gyrA96 thi-1 relA1 ΔlacU169 (φ80lacZΔM15); K-12 derivative | 12 |
| SF100 | F− ΔlacX74 galK thi rpsL (strA) ΔphoA(pvuII) ΔompT | 2 |
| FTL10 | ΔyidC attB::(araC+ PBADyidC+); Kanr | 13 |
| B. subtilis strains | ||
| 168 | trpC2 | 25 |
| ATCC 6633 | Subtilin producer | 7 |
| pNZ8900 | 168 amyE::spaRK; Kmr | 3 |