FIG. 2.
RT-PCR analysis for M. genitalium genes MG_454 (A), MG_453 (B), and MG_455 (C) from total RNA isolated from M. genitalium wild-type (Wt) and ohrMg mutant (ΔMG_454) strains. PCR products were separated on 1.0% agarose. M, molecular-size marker; DNA, M. genitalium genomic DNA as a template for PCR; RT+, product generated in the presence of reverse transcriptase (Superscript II; Invitrogen) as a template for PCR; RT−, product generated in the absence of reverse transcriptase as a template for PCR. The reaction RT− was done to prove the absence of DNA in total RNAs used for reverse transcriptions.