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Functional antagonism of Smac mimetics against XIAP BIR3 in a cell-free functional assay. dATP and cyctochrome c were added into cell lystates to activate caspase-3/7 and recombinant XIAP BIR3 protein at 500 nM effectively inhibited casapse activity. Smac mimetics dose-dependently recovered the activity of casapse-3/-7. The caspase activity data at 60 min was used (Supporting Information).
