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. 2009 Nov 17;5:328. doi: 10.1038/msb.2009.78

Figure 4.

Figure 4

Persistent Clb2 after mitotic exit eliminates G1 mating factor sensitivity. A bar1Δ strain (BD127b-9D) was released from cdc20 arrest into α-factor-containing medium (A) without or with (B) a Clb2kd–YFP pulse. Merged YFP, mCherry, and DIC channels are shown. Unbudded cells (black), complete (red) or partial (blue) cytokinesis, and rebudding (green) are plotted versus time post-release for unpulsed cells (A) or versus Clb2kd–YFP concentration 60-min post-release for pulsed cells (B). (C) Cells bearing Myo1–mCherry and GFP fused to the Cln2 degron under the control of the CLN2 promoter (BD143a-21C) were released from cdc20 arrest into α-factor-containing medium with or without a pulse of untagged Clb2kd. Merged GFP, mCherry and DIC channels are shown at 0 and 75 min post-release.