Fig. 2.

The DN-CKIδ/ε lengthened the period of bioluminescence (PER2:Luc) rhythms in MEFs. (A–C) MEFs were infected with adenovirus expressing DN-CKIδ (A), DN-CKIε (B,) or wt CKIε (C) for 2 h, serum-shocked for 2 h and placed into the real-time luminometer. The 3XFLAG tag added to the N-termini of wt and DN kinases was used to ensure similar expression among different adenoviruses. ½× represents half titer of 1×. The numbers are shown as mean ± SEM of triplicate samples. The results are representative of several experiments. *, P < 0.05; **, P < 0.01.