Fig. 2.

Involvement of Gq, intracellular calcium, and CaMKIIα in the D1-D2 heteromer signaling pathway in striatal neurons. (A) Inhibition of 100 nM SKF 83959-triggered intracellular Ca²⁺ mobilization by Gq-specific inhibitor YM 254890 (YM, 100 nM). (B) Time-dependent trafficking of endogenous Gq, after treatment with vehicle (control, 0 min) or SKF 83959 (100 nM) for 2 and 5 min. (C) Intracellular Ca²⁺ mobilized by SKF 83959 with or without U73122, inhibitor of PLC. (D) Increase in phosphoCaMKIIα (pCaMKIIα) following treatment with vehicle or 100 nM SKF 83959 for 2 min or 5 min in cytosol and nucleus. (E) Quantification of total pCaMKIIα fluorescence (cytosolic + nuclear) over the basal. (F) Quantification of pCaMKIIα fluorescence in the nuclear compartment over the basal level. Data represent mean +SEM of values from at least three different experiments. *, significant difference from control (P < 0.01).