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. 2009 Nov 30;106(50):21377–21382. doi: 10.1073/pnas.0903676106

Fig. 4.

Fig. 4.

D1-D2 receptor heteromer localization and enhancement of BDNF expression in rat nucleus accumbens. (A) Confocal FRET analysis of D1 and D2 receptor interaction in rat nucleus accumbens shell region. Anti-D2-Alexa 350 and anti-D1-Alexa 488 were used as donor and acceptor dipoles. Analysis shows processed FRET (pFRET) and distances separating the two receptors. FRET signal was detected only in neurons coexpressing D1 and D2 receptors. (B) Microdomains [regions of interest (ROIs)] within neurons coexpressing D1 and D2 receptors (Bi, Upper) or expressing D2 exclusively (Bii, Lower) were analyzed. Average FRET efficiency (E) and distance between donor and acceptor are shown. A distance ≥10 nm indicates no FRET. (C) BDNF expression in rat nucleus accumbens core and shell regions from animals treated with vehicle (saline), SKF 83959, or SKF 83822. (D) Quantification of BDNF-positive neurons (Left) and densitometry within each neuron (Right) from animals treated with vehicle (saline), SKF 83959, or SKF 83822 were assessed. Results represent mean +SEM of values from 4–5 rats/group.