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. 2009 Dec 1;106(50):21365–21370. doi: 10.1073/pnas.0911782106

Fig. 4.

Fig. 4.

RORα1, RORα4, and REV-ERBα specifically bind to a putative RORE of the Il-6 promoter. EMSA was performed with radiolabeled double-stranded oligonucleotides that contained the putative RORE of the human Il-6 (hIL-6) promoter. Radiolabeled hIl-6 RORE oligonucleotides were incubated with RORα1 (Left, lanes 3–9), RORα4 (Center), REV-ERBα (Right), no proteins (Left, lane 1) or with un-programmed reticulocyte (UP) lysate as control (Left, lane 2). Competition assays were carried out by incubating radiolabeled hIl-6 RORE oligonucleotides with RORα1 (Left, lanes 4–9), RORα4 (Center, lanes 2–7), or REV-ERBα (Right, lanes 2–7) in the presence of unlabeled native or mutated hIl-6 RORE oligonucleotides at 10-, 50-, or 100-fold molar excess. Arrows indicate migration of double-stranded oligonucleotides (Lowerr) and DNA-protein complexes (Upper).