Fig. 2.

Comparison of the CL distribution profiles for wild-type and mutant amylopectins. After purification on a gel filtration Sepharose CL-2B column, amylopectin was debranched with isoamylase and pullulanase. The resulting glucans were analyzed by CE. The relative proportions for each glucan in the total population are expressed as a percentage of the total number of chains. A and C are, respectively, corresponding to the CL distribution obtained from wild-type amylopectin without and with β-amylase pretreatment. B and D are to the corresponding mutant amylopectin samples. The unbroken lines on B and D correspond to the difference plots obtained by subtracting the mutant CLD to the wild-type CLD.