Fig. 6.

YvrHa interacts with the amino terminus of YvrI. (A) BACTH analysis of interactions between YvrHa and either full-length YvrI or amino- and carboxyl-terminal segments of YvrI (Fig. S1). (B) In vitro pull-down assay using NHS-conjugated YvrHa or a control protein (lysozyme). Binding reactions included either full-length YvrI or the amino- or carboxy-terminal segment of YvrI. Arrows indicate bound amino-terminal fragment of YvrI. (C) In vitro pull-down assay testing interaction between immobilized YvrHa and either YvrI, SigA, or SigM. All test proteins were added to binding reactions to a final concentration of ≈1 μM.