Figure 1.

Purity of A. suum QFR at different stages of purification. Samples were separated by SDS–PAGE and the gel was stained with Coomassie Blue. The positions of molecular-weight markers are indicated on the left (in kDa) and the four subunits of A. suum QFR (Fp, flavoprotein subunit; Ip, iron–sulfur cluster subunit; CybL, cytochrome b large subunit; CybS, cytochrome b small subunit) are labelled on the right. Lane 1, whole mitochondria; lane 2, supernatant obtained after ultracentrifugation of the detergent extract; lane 3, pooled fractions from the DEAE Sepharose FF column; lane 4, pooled fractions from the Source 15Q column.