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. Author manuscript; available in PMC: 2010 Dec 31.
Published in final edited form as: J Immunol Methods. 2009 Sep 23;351(1-2):55–61. doi: 10.1016/j.jim.2009.09.004

Figure 1. Assessment of the b96.11-biotin/streptavidin assay.

Figure 1

Three serum samples were analyzed for the presence of GAD65Ab-specific anti-Id in the b96.11-biotin/streptavidin assay. Samples were with b96.11-biotin/streptavidin-agarose complexes and subsequently separated by centrifugation. GAD65Ab were measured in serum samples prior to and after absorption by PAS-RBA (white and black, respectively), and streptavidin-agarose-RBA (grey columns). Samples that did not contain any human serum, but otherwise underwent the same procedure were analyzed as control samples to ascertain that GAD65Ab did not leak off the beads (blank). GAD65Ab levels are shown as index, the figure represents three independent experiments.