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. Author manuscript; available in PMC: 2010 Dec 31.
Published in final edited form as: J Immunol Methods. 2009 Sep 23;351(1-2):55–61. doi: 10.1016/j.jim.2009.09.004

Figure 4. Temperature dependency and specificity of ECL.

Figure 4

A: Seven serum samples (1–7) and buffer blanks contained PBS instead of serum were analyzed at different temperature incubations in the ECL assay. Samples were first incubated for 10 minutes at 55°C (white), 42°C (light grey), or 37°C (dark grey), then for 30 minutes at 37°C, and finally for 15 minutes at room temperature. A fourth set of the samples underwent the assay at room temperature only (black). Anti-Id levels are shown as relative index.

B: serum samples (n=40) were tested for specificity of binding in the ECL assay in the absence of competitor (black circles), and in presence of unlabeled rFab b78 (white circles), or unlabeled rFab b96.11 (black diamonds). Anti-Id levels are shown as relative index. Median binding is indicated.